Figure 8
From: Host Double Strand Break Repair Generates HIV-1 Strains Resistant to CRISPR/Cas9
Three CRISPR gRNAs targeting TAR have similar effects on HIV-1 replication.
Wild type human CD4+ T cell line SupT1 and derivatives expressing CRISPR Cas9 and three gRNAs targeting HIV-1 TAR were infected with (a,b) strain NL4-3 or (c,d) strain R7. Data from infection of four different cell lines are shown for direct comparison of the efficiencies of the TAR gRNAs. All three TAR gRNAs suppress HIV-1 replication equally. (a,c) HIV-1 replication was measured by the release of p24 to the culture supernatants. (b,d) Cellular viability was measured by trypan blue exclusion. Error bars indicate the standard deviation between three infections.
