Figure 1: Isolation of Ae1a.

Chromatogram resulting from fractionation of crude Augacephalus ezendami venom using C18 RP-HPLC. The dashed line indicates the gradient of solvent B (90% acetonitrile/0.05% TFA). The shaded peak containing the active peptide Ae1a was lethal when injected into Drosophila melanogaster. The inset shows the MALDI MS spectrum of purified Ae1a.