Figure 2: Production of recombinant Ae1a. | Scientific Reports

Figure 2: Production of recombinant Ae1a.

From: Molecular basis of the remarkable species selectivity of an insecticidal sodium channel toxin from the African spider Augacephalus ezendami

Figure 2

RP-HPLC chromatogram showing final purification of rAe1a (shaded peak). The dashed line indicates the gradient of solvent B (90% acetonitrile/0.043% TFA). The inset is an SDS-PAGE gel showing cleavage of the MBP-Ae1a fusion protein with TEV protease. Lanes 1 and 2 correspond to pre- and post-cleavage samples, while lane M contains molecular weight markers (masses indicated in kDa). The bands corresponding to uncleaved and cleaved fusion protein are indicated. Note that this image is cropped from the original gel scan, which is shown in its entirety as Supplementary Figure 1, but is otherwise unmodified.

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