Figure 3
From: Morphologically and Functionally Distinct Lipid Droplet Subpopulations
Separation of lipid droplet subpopulations from Huh7 cells by size.
LDs from Huh7 cells were fractionated by size using differential centrifugation (Materials and Methods). Four LD fractions were collected. (A) EM to determine LD size. Huh7 cells and the four LD fractions were fixed, dehydrated and infiltrated and finally embedded for ultra-thin sectioning. The 70 nm ultra-thin sections were prepared and the ultra-structures were analyzed by EM. (B) Protein composition of the subpopulations. Proteins of the four LD fractions were extracted with chloroform:acetone (300 μl:700 μl) and analyzed by silver staining and Western blotting (equal amount of protein per lane) with the indicated antibodies.
