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Figure 2

From: Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration

Figure 2

10E8 peptide specific antibody responses in serum and intestinal washes and antibody-secreting cells in bone marrow induced by oral immunization with ST-10E8.

(a–c) Mice (5 per group) were orally primed with attenuated ST-10E8, ST or PBS and boosted with 10E8 peptide for two times and then 10E8 peptide specific serum IgG (a), intestinal wash sIgA (b) and serum anti-HIV-1 SF162 neutralizing antibody response (c) were detected. (d–f) Specific antibody secreting cells (ASCs) and total IgG ASCs at day 14 after the final boost were measured by B cell ELISpot assay. The spots of five mice in each group (d), the specific ASC (e) and total IgG ASC in 106 bone marrow cells (f) were shown. (g) Correlation between specific antibody titer and the specific ASCs in bone marrow (BM) was shown. NP, neutralization percentage. R, correlation coefficient. Data shown as mean ± SD of 5 mice in each group.

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