Figure 3 | Scientific Reports

Figure 3

From: Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration

Figure 3

Total IgG, IgG subclasses and cytokine levels in sera of immunized animals.

(a) Serum total IgG levels at day 14 after the final 10E8 peptide boost were measured by quantitative ELISA. The correlations between the serum total IgG concentration and total IgG ASC in BM (b) and the specific antibody titer (c) were shown. (d) IgG subclasses at day 14 after the final 10E8 peptide boost were analyzed by quantitative ELISA. (e) The Th1/Th2 bias of immunity was shown as the ratio of IgG2a to IgG1 in concentration. (f) The levels of Th1 cytokines (IL-10, IFN-γ, TNF-α) and Th2 cytokines (IL-4, IL-6, IL-21) in sera of immunized mice were detected by quantitative ELISA. R, correlation coefficient. Data shown as mean ± SD of 5 mice in each group. The asterisk indicates a significant difference versus PBS control (*P < 0.05).

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