Table 1 Population genetic diversity in the four selected CHIKV strains.

From: Importance of mosquito “quasispecies” in selecting an epidemic arthropod-borne virus

nt position

nt change

Gene

aa change

Frequency of mutations (%)

Ae. aegypti

Ae. albopictus

P10_AE7

P10_AE14

P10_AL7

P10_AL14

562

A -> G

nsp1

162 (L)#

 

99.9

  

978

C -> T

nsp1

T301I

 

99.8

  

1017

C -> T

nsp1

T314M

 

100

  

1851

G -> A

nsp2

A57T

99.9

   

2635

C -> T

nsp2

318 (T)#

99.8

   

3210

A -> C

nsp2

K510T

99.6

99.3

99.7

 

3734

A -> G

nsp2

I685D

   

99.9

4424

G -> A

nsp3

G117R

   

99.9

4897

C -> T

nsp3

274 (T)#

 

99.8

  

5942

C -> T

nsp4

P93S

97.2

 

93

 

6157

C -> T

nsp4

164 (F)#

 

98.7

  

6724

C -> T

nsp4

353 (F)#

   

100

7113

G -> A

nsp4

C483Y

99.9

   

8785

G -> A

E2

G82R

  

12.2*

 

9190

A -> G

E2

I217V

 

99.9

  

9286

G -> A

E2

G249R

 

98.8

  
  1. nt, nucleotide; aa, amino-acid; #no amino-acid change, synonymous mutation; *minority variant not found in the consensus sequence; P10_AE7, 10th passage between HFF cells and Ae. aegypti initiated with saliva collected at 7 dpi; P10_AE14, 10th passage between HFF cells and Ae. aegypti initiated with saliva collected at 14 dpi; P10_AL7, 10th passage between HFF cells and with Ae. albopictus initiated with saliva collected at 7 dpi; P10_AL14, 10th passage between HFF cells and Ae. albopictus initiated with saliva collected at 14 dpi. The DRC_2000_HFF was used as the reference genome.