Figure 2
From: Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores
SS-nanopore detection of 5 hmC in mouse genomic DNA (a) Scheme showing preparation of genomic DNA for the SS-nanopore assay. (b) Gel electrophoresis of fragmented mouse genomic DNA (‘F’) and two low-molecular weight DNA ladders (L1 and L2, band labels are in bp). (c) Event rate vs. applied voltage measured for genomic DNA treated as described in (a) at total DNA concentrations co of 1 μM (red triangles) and 475 nM (red circles) in the presence of excess MS with 9.1 and 9.3 nm diameter nanopores, respectively. Black data points are a negative control of the same material (no MS). Solid lines are exponential fits to the data.
