Figure 3
From: Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores
Quantification of 5 hmC content by SS-Nanopore assay (a) Event rate vs. concentration (400 mV) for 75 bp monobiotinylated dsDNA with excess MS. Solid line is a linear fit to the data and shaded region represents error of fit. Solid red lines show experimentally-derived event rate values for mouse genomic DNA, biotin-labeled at 5 hmC sites, and in the presence of excess MS, for i: NP1 (co = 1 μM, diameter 9.1 nm) and ii: NP2 (co = 475 nM, diameter 9.3 nm). (b) Quantification of 5 hmC/C ratio R determined for the values in (a) and comparison with LC-MS/MS measurements of the same material. Error bars for nanopore measurements represent error of the fit from (a).
