Figure 1
From: Heritable genome editing with CRISPR/Cas9 induces anosmia in a crop pest moth
S. littoralis Orco (SlitOrco) gene structure and guide RNA positions.
SlitOrco consists of 10 exons (grey boxes, E) and 9 introns. The number of amino acids encoded by each exon is indicated in the box. Two gRNAs, cr104 and cr105, were designed in exon 2 (E2) and one, cr106, in exon 4 (E4). Exon 2 and 4 sequences are detailed (underligned parts) showing positions of gRNAs (in blue ; PAM in italic and in box). Primers used for PCR amplification in the genotyping assay are in red italic.
