Figure 2
From: Heritable genome editing with CRISPR/Cas9 induces anosmia in a crop pest moth
Comparison of the activity of the different guide RNAs (gRNAs).
Three gRNAs (cr104, cr105, cr106) were co-injected with the Cas9 protein in different egg batches (9 batches illustrated for cr104 and 3 batches illustrated for each of the two other guides). Batches of emerging larvae were genotyped by the T7EI assay following genomic DNA extraction and PCR amplification of an Orco fragment (for fragment sequences and primers, see Fig. 1). DNA modification could be obtained only for cr104 and in all egg batches, as revealed by the gel pattern: the highest band corresponds to the wild type gDNA amplification and the two small bands (arrows) result from T7EI action. Water injected larvae (H2O) and no template (−) controls are shown for each primer combination. The ladder used is the Quick-load 2-Log DNA Ladder (0.1–10.0 kb, BioLabs).
