Figure 2
From: The role of dimensionality in neuronal network dynamics
Cellular morphology of 2D and 3D cultures.
(a) SEM image of a 2D graphene film. Darker areas present an higher number of layers compared to the brighter one; as shown in the inset the surface is flat. (b) SEM image of a 3D Graphene Foam scaffold (3D-GF); the surface presents ripples, as shown in the inset. (c) Hippocampal culture at 7 DIV on 2D Glass stained for β-tubulin III (TUJ1, red), glial fibrillary acidic protein (GFAP, green) and Hoechst 33342 nuclear stain (blue). (d) The same as (c) but for 3D-GFs. (e) Proportion of neurons (TUJ 1-positive) and glia (GFAP-positive) among different substrates tested. TUJ 1- and GFAP-negative cells are referred to as “other”. (f,g) GFAP staining of astrocytes on 2D G and 3D-GF, respectively. (h) Percentage of astrocytes with processes for the different substrates tested (**p < 0.01 ***p < 0.001 One-way ANOVA, Holm-Sidak post-hoc test). (i,j) Neuronal cultures on 2D G and and 3D-GFs respectively. Cells were stained for MAP2 (green), GABA (red) and Hoechst 33342 nuclear marker (blue). (k) Percentage of GABAergic inhibitory neurons for three conditions tested. The images are the projections of a 20 μm z-stack for 2D samples and 35–50 μm for the 3D cultures, acquired with 0.5-μm slice spacing. Scale bar, 50 μm.
