Figure 3
From: The role of dimensionality in neuronal network dynamics
The spontaneous activity of 3D networks is more synchronous.
(a,b) Neuronal cultures loaded with 4 μM Fluo-4-AM calcium indicator on 2D Glass and 3D-GF, respectively. (c,d) Glial cells on 2D G and 3D-GF respectively. Coloured circles indicate the ROIs where calcium waves were obtained. (e,f) Calcium transients on 2D Glass and 3D-GF, respectively, for 4 selected neurons. (g) Calcium waves from glial cells on 2D G (top traces) and 3D-GFs (bottom traces); arrows indicate signals from neurites projecting over glial cells. (h) Raster plot of 2D culture on glass and (i) 3D culture on 3D-GFs for 22 selected traces. (j) Cumulative count of the Inter Events Interval (IEI) for neuronal cultures grown on 2D Glass, 2D G and 3D-GF (**p < 0.01 ***p < 0.001 ANOVA on ranks, Domm’s post-hoc test). (k) Cross-correlation matrices of calcium transients 
in 2D and 3D neuronal networks. (l) Cross-correlation matrices of slow calcium signals 
for the same two conditions. (m) Mean correlation coefficient of the calcium transients and slow calcium signals (***p < 0.001 ANOVA on ranks, Domm’s post-hoc test). Scale bar, 50 μm.
