Figure 5
From: The role of dimensionality in neuronal network dynamics
High connectivity leads to HS regime.
(a) Fluorescent image of a neuronal culture grown on a 3D-GF loaded with Fluo-4 AM; a crossing neurite (inset) and two examples of ROIs (black circles) are shown. (b) Optical traces from the crossing neurite shown in a (red trace) and from two neighbouring neurons (black traces) obtained from the two ROIs indicated in a. (c) Fluorescence image of a neuronal culture grown on a 3D-GF where neurons cross and fill a pore of the scaffold with both neurites and cell bodies. Cells were stained for the somatodendritic neuronal marker MAP2 (red), axonal marker SMI 312 (green) and Hoechst 33342 nuclear stain (blue). (d) Example of a highly connected network as in c but loaded with Fluo-4 AM exhibiting the HS regime. (e) 24 superimposed optical traces (3 of them are shown separately in the bottom part) obtained from the 3D neuronal network shown in d; the rising phase of calcium transients is almost perfectly synchronized, as shown in the insets. (f) Time evolution of the correlation coefficient cc computed over a bin width of 2 minutes for 3D neuronal networks in the MS and HS regime and for 2D neuronal networks. (g–i) are examples of optical traces for each type of regime with the same colour code as in (f). Scale bar, 50 μm.
