Table 1 Phenotypes of donor and chimeric embryos 1 .

From: Germline replacement by blastula cell transplantation in the fish medaka

Sample

 

Embryos observed

Pigmented, n (%)

Lr-positive, n (%) 2

Vg-positive PGC

Total PGCs predicted 4

n (%) 2

PGC 3

%

PGC3

BGR donor

normal

31

31 (100)

20 (64.5)

21 (67.7)

33.1 ± 2.93

100

33.1 ± 2.93

dnd RNA

30

30 (100)

20 (66,6)

20 (66.7)

82.5 ± 5.42

100

82.5 ± 5.42

Chimera 5

Class I

36

36 (100)

21 (58.3)

17 (47.2)

1.41 ± 0.51

63.5

1.6 ± 0.8

Class II

32

32 (100)

18 (56.2)

26 (81.2)

2.08 ± 0.74

92.4

2.7 ± 1.3

Class III

37

37 (100)

21 (56.8)

30 (81.1)

2.07 ± 0.78

Class IV

24

24 (100)

18 (75.0)

19 (79.2)

2.21 ± 0.71

  1. 1Vg was observed at stages 18–23 when PGCs were positioned bilaterally to somites and easily countable. Melanocyte and Lr were observed from 5 dpf onwards. All embryos are positive for pigmentation.
  2. 2Comparisons between embryos observed and positive for Lr or Vg.
  3. 3Number of heterozygous VgPGCs per embryos presented as means ± sd.
  4. 4Percentages of donor PGC-containing embryos and numbers of total PGCs. These values in donor embryos are the same as experimentally determined, and in chimeras were predicted from the genotype of BGR embryos (see Fig. 1) and the bimodal distribution by using 1 PGC including 0.66 VgPGC [2/3(33.1 PGCs) per 1000-cell donor blastula; class I) and 2.5 PGCs including 1.65 VgPGCs [2/3(82.5 PGCs) per 1000-cell donor blastula; classes II-IV] as the input numbers of PGCs within 30 donor blastula cells transplanted.
  5. 5For chimera classes see Fig. 2.
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