Figure 8: Activation of IRF3 together with SAMHD1 upregulation in virus infected cells. | Scientific Reports

Figure 8: Activation of IRF3 together with SAMHD1 upregulation in virus infected cells.

From: Interferon regulatory factor 3 is a key regulation factor for inducing the expression of SAMHD1 in antiviral innate immunity

Figure 8

PAMs (A) and MARC-145 cells (B) infected with HP-PRRSV at an MOI of 5 were harvested at indicated times, and used for western blot analysis. Changes in TBK1, IRF3 and SAMHD1 expression were detected by western blotting. β-actin was used as a loading control. (C) Cell lysates of PAMs infected with HP-PRRSV were collected at 12 h and 16 h p.i. and analyzed by Native SDS-PAGE to visualize dimers of IRF3. (D) MARC-145 cells and PAMs were infected with HP-PRRSV for 2, 6 and 12 h. Nuclear proteins were extracted and the nuclear translocation of IRF3 and SAMHD1 expression were analyzed using Western blotting. PCNA was used as a loading control. Expression levels of SAMHD1 compared to β-actin or PCNA are shown. Uncropped images of blots are shown in Supplementary Figure 8.

Back to article page