Figure 6: Irisin expression and secretion is decreased in obese mCaROCK1 mice.

(a) Muscle mRNA level of myockines were examined by quantitative real-time PCR. (b) Plasma level of Irisin, IL-6 and Myostatin (MSTN) were measured by Elisa assay. Data are presented as the mean ± SEM, n = 3–5, *p < 0.05, mCaROCK1 vs. CTL.