Figure 7: Irisin replenishment improves obesity and insulin resistance in mCaROCK1 mice.

(a) Blood irisin concentration was determined by ELISA assay. (b) Glucose tolerance was improved in mCaR1 mice after irisin treatment. (c) After irisin treatment, fat mass was measured by echo-MRI, data is represented as percentage of bodyweight (BW). (d) Oxygen consumption (VO2) analyzed by indirect calorimetry in mCaROCK1 mice treated with or without irisin. (e) Respiratory exchange ratio of mCaROCK1 and CTL mice. (f) Heat generation was measured in mCaR1 mice treated with or without irisin. All data are presented as the mean ± SEM, n = 8, *p < 0.05. mCaROCK1 + Irisin vs. mCaROCK1.