Figure 8: Irisin stimulates UCP1 expression in WAT in mCaROCK1 mice.

(a) Western blot revealed that UCP1 was significant increase in WAT of mCaROCK1 mice after irisin treatment (mean ± SEM, n = 5, *p < 0.05, **p < 0.01, mCaROCK1 + Irisin vs. mCaROCK1). (b) Immunohistochemistry of UCP1 in WAT of mCaROCK1 mice treated with or without irisin (DAB staining). (c) Western blot indicated that Perf-1 was significant increase in WAT of mCaROCK1 mice after irisin treatment (mean ± SEM, n = 5, *p < 0.05, **p < 0.01, mCaROCK1 + Irisin vs. mCaROCK1). (d) Immunohistochemistry of Perf-1 in WAT of mCaROCK1 mice treated with or without irisin (DAB staining).