Figure 9: Constitutive ROCK1 activation suppresses Irisin expression via down-regulation of PGC1a. | Scientific Reports

Figure 9: Constitutive ROCK1 activation suppresses Irisin expression via down-regulation of PGC1a.

From: ROCK1 reduces mitochondrial content and irisin production in muscle suppressing adipocyte browning and impairing insulin sensitivity

Figure 9

(a) C2C12 cells were transfected with control (empty vector), or overexpression vector encoding constitutive activation ROCK1 (CaROCK1, 130 kD), or kinase mutant ROCK1 (mutROCK1, 130 kD). After 24 h, endogenous ROCK1 (total ROCK1) and CaROCK1 or mutROCK1 were accessed by western blot. (b) PLG3 LUC reporter plasmid containing FNDC5 (precursor of irisin) promoter were co-transfected with control vector or CaROCK1 or mutROCK1 into C2C12, LUC activities were measured after 24 h of transfection (mean ± SEM, n = 5, *p < 0.05, vs. Vector). (c) mRNA levels were accessed with qRT-PCR in C2C12 cells transfected with empty vector; CaROCK1 or mutROCK1 (mean ± SEM, n = 3, *p < 0.05, vs. Vector). (d) PGC-1a level were examined by western blot in C2C12 cells transfected with plasmids as indicated. (e) Western blot analysis of PGC-1a in muscles of CTL littermates and mCaROCK1 mice (mean ± SEM, n = 5, *p < 0.05, vs. CTL).

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