Figure 2

Calculated (a) average microtubule width and (b) average continuity of microtubules for each fluorophore, organized by observed quality. Average width and continuity measurements are graphed as the mean ± standard deviation of n = 15 measurements per image of the corresponding fluorophore. Images were collected at the following fluence rates: 0.28 kWcm⁻² for the 488 nm laser line (blue points), 0.49 kWcm⁻² for the 561 nm laser line (green points) and 1.11 kWcm⁻² for the 647 nm laser line (red points).