Figure 6: CALI of complex II sensitizes C. elegans to mild stress.

Wild-type (+), tm1081; inactive, and tm1081; active strains were: (A) illuminated as L1 larvae for the indicated period and cultured on NGM plates containing 200 μM paraquat. 96-hour survival is plotted relative to the zero-illumination baseline. Baseline survival on 200 μM paraquat was wild-type (+), 78 ± 4%; tm1081; inactive , 68 ± 4%; tm1081; active, 60 ± 3%. Data are mean ± SEM. N = 11–13. An “N” is considered an independent trial of >50 worms. *Adjusted P < 0.05 vs the no light or vs the light-matched inactive counterpart (2-way ANOVA with Sidak multiple comparisons test). (B) Synchronized L4 were illuminated for the indicated time and cultured on NGM plates seeded with OP50 containing 1 μM FCCP. Viability was assessed 1 day later and plotted relative to zero-illumination baseline. Baseline survival on 1 μM FCCP was wild-type (+), 99 ± 1% survival; tm1081; inactive, 93 ± 3%; tm1081; active, 94 ± 2%. Data are mean ± SEM. N = 9. An “N” is considered an independent trial of >50 worms. *Adjusted P < 0.05 vs the no light or vs the light-matched inactive counterpart (2-way ANOVA with Sidak multiple comparisons test). Data were normalized to wild-type (+) to account for miniSOG-independent effects of blue light. Alleles are abbreviated as outlined in Fig. 1.