Figure 3 | Scientific Reports

Figure 3

From: Receptor tyrosine kinase EphA7 is required for interneuron connectivity at specific subcellular compartments of granule cells

Figure 3

siRNA-mediated knockdown of EphA7 reduces gephyrin cluster density in hippocampal neurons.

(a–g) Hippocampal neurons were cotransfected with pEGFP-N1, a scrambled control siRNA (siCTR) or siRNA specific for EphA7 (siEphA7), for FGFR1 (siFGFR1), or for ACVR2A (siACVR2). Cultures were fixed and stained for expression of gephyrin (a,c), the γ2 sub-unit of GABAA receptors (b,d) or GAD65 (e,f). Transfected cells were identified by green EGFP fluorescence. Magnifications of insets in (a–f) are shown in (a’–f’). All images were rendered using Imaris software. Scale bars, 10 μm. (g) Densities of gephyrin, GAD65, PSD95 and γ2 subunit punctae were quantified per 20 μm dendritic segment, n = 30; **p < 0.01; ***p < 0.0001; ANOVA, Fisher-PLSD. For knockdown efficiency of EphA7 siRNA see supplementary Figure S2.

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