Figure 5
Evaluation of tumor composition in 3D.
(a) Representative maximum projection images of HCC4011 (red), HCC4011R (green) and admixed (1:1 ratio, HCC4011:HCC4011R) spheroids on days 1 and 8 with and without erlotinib (1 μM) treatment. Spheroids were dissociated on days 0,7,10 and 14 and monolayer cultures were stained with Hoechst and DRAQ7 and imaged to generated live and dead cell counts. (b) Average cell count from dissociated tumors was plotted over time for HCC4011, HCC4011R and admixed tumors. Spheroids were treated with erlotinib on day 7. (c) Bar graph depicting average quantitative changes in HCC4011 and HCC4011R subpopulations (live and dead cell counts) in admixed spheroids over time in response to erlotinib treatment (1 μM).
