Figure 2
From: Evaluation of a Micro-Optical Coherence Tomography for the Corneal Endothelium in an Animal Model
Histology images compared to μOCT scan images of the normal mouse cornea.
(A) Cross-sectional histology sections using H&E stain (20x magnification, scale bar = 20 μm). *Indicates Bowman’s layer. Epithelium lies above the Bowman’s layer. Arrow indicates Descemet membrane, corneal stromal layer lies in between. Endothelial cell layer can be seen just below the Descemet membrane. (B) Cross-sectional B-scan μOCT images were able to delineate the corresponsing distinct layers of the cornea with similar resolution to histology images i.e. *Bowman’s layer, collagen fibrils in stroma, Descemet membrane (arrow) and endothelial cell layer below (scale bar = 40 μm). Epithelium lies above the Bowman’s layer. Arrow indicates Descemet membrane, corneal stromal layer lies in between. Endothelial cell layer can be seen just below the Descemet membrane. (C) Endothelial cell layer histology image using Alizarin S Red (20x magnification, scale bar = 20 μm). (D) En face μOCT scan of the normal endothelial cells without artifacts from histology fixing and staining (scale bar = 40 μm) was able to detect the presence of nuclei and villi.
