Table 1 Selection Parameters.
From: Highly Multiplexed RNA Aptamer Selection using a Microplate-based Microcolumn Device
Round | Number of Binding Cycles | Binding Step Parameters | Washing Step Parameters | Elution Step Parameters |
|---|---|---|---|---|
1 | 1 | 1 mL at 1 μL/min | 3 mL at 1 μL/min | 400 μL at 50 μL/min |
2 | 2 | 1 mL at 10 μL/min | 1 mL at 1 mL/min, 2 mL at 70 μL/min | 400 μL at 50 μL/min |
3 | 3 | 1 mL at 10 μL/min | 1 mL at 1 mL/min, 2 mL at 70 μL/min | 400 μL at 50 μL/min |
4 | 4 | 1 mL at 10 μL/min | 1 mL at 1 mL/min, 2 mL at 70 μL/min | 400 μL at 50 μL/min |
