Figure 3: Inhibition of autophagy by siRNA induced more cell death than VNP20009 treatment.
From: Chloroquine enhanced the anticancer capacity of VNP20009 by inhibiting autophagy
(a) Western blots of ATG5 protein expression from B16F10 lysates after transfection of siRNA with potential for silencing ATG5. (b) Protein expression of ATG5 was analyzed by ImageJ. Data are presented as mean ± SD. **p < 0.01. (c) Protein expression of LC3-II after infected with VNP20009 in the wild type or ATG5 knock out cells. (d) Protein expression of LC3-II was analyzed by ImageJ. Data are presented as mean ± SD. *p < 0.05, **p < 0.01. (e) The wild type or ATG5 knock out cells were infected with VNP20009 at different MOI. Cell death was analyzed using flow cytometry. Data are presented as mean ± SD. *p < 0.05, **p < 0.01. (f) The wild type or ATG5 knock out cells were infected with VNP20009 at MOI of 10:1 at indicated time. Cell death was analyzed using flow cytometry. Data are presented as mean ± SD. *p < 0.05, **p < 0.01.
