Figure 2
From: Protein Kinase C Epsilon Promotes Cerebral Ischemic Tolerance Via Modulation of Mitochondrial Sirt5
PKCε Increases SIRT5 Desuccinylase Activity via Mitochondrial Nampt.
Mitochondria from rat neuronal-astrocyte cortical cultures isolated 48 hours following ΨεRACK (100 nM) treatment or IPC were exposed to DMSO, FK866 (Nampt inhibitor, 50 μM), or were not treated. (a) Increased desuccinylation activity following ΨεRACK treatment was blocked with mitochondrial Nampt inhibition. (b–d) Western blot analyses of lysine succinylation and SIRT5 in isolated cortical mitochondria. Gels were run under the same experimental conditions. Blots displayed are cropped and full-length blots are presented in Supplementary Figure S1. (c) PKCε activation decreased lysine succinylation which was reversed with exposure to FK866. (d) ΨεRACK treatment increased SIRT5 protein levels. (e) Increased desuccinylation activity following IPC was blocked by exposure to the PKCε inhibitor εV1–2 (100 nM) or FK866 in mitochondria. *p < 0.05.
