Figure 6
From: Protein Kinase C Epsilon Promotes Cerebral Ischemic Tolerance Via Modulation of Mitochondrial Sirt5
SIRT5 is Required for PKCε-mediated Protection Against OGD-induced Cell Death.
(a–d) Neuronal-astrocyte cultures obtained from WT or SIRT5−/− mouse cortex. (a) Fluorescence images of cultures at 16 h of reperfusion after lethal OGD. Cultures were stained with the cell-permeable nuclear marker Hoechst (blue) and Yo-pro (green) which is permeable to apoptotic and necrotic cells. ΨεRACK treatment significantly decreased Yo-pro staining in WT cultures whereas ΨεRACK treatment in SIRT5−/− cultures had no protective effect; scale bar 100 μm. (b) Quantification of images in a. (c,d) Cell death measured by LDH release at 16 h and 48 h of reperfusion after OGD. (c) ΨεRACK treatment decreased LDH release at 48 h of reperfusion after lethal OGD in WT cultures, while SIRT5−/− cultures displayed no significant changes in LDH release. (d) LDH release in WT and SIRT5−/− 48 h following sham OGD treatment show no significant difference. * p < 0.05, **p < 0.01.
