Figure 5

Electroporation of the expression vectors with astrocyte-specific promoter.

(a–e) The electroporation of pGLAST-DsRed, in which DsRed was driven by rat GLAST promoter. GFP expression vector with CAG promoter was co-transfected with pGLAST-DsRed. After the focal electroporation, DsRed expression is detected in GLAST-immunoreactive cells. (f–j) The electroporation of pGFAP-LacZ in which ß-galactosidase was driven by mouse GFAP promoter. GFP expression vector with CAG promoter was co-transfected with pGFAP-LacZ. After the focal electroporation, LacZ expression was detected in GFAP-immunoreactive cells. (k–m) Doxycycline-dependent gene expression in astrocytic cells. The adult SVZ was co-transfected with pGFAP-TetON and the reporter plasmid (pTRE-ZsGreen) via focal electroporation (k). ZsGreen expression in an astrocytic cell (a yellow arrow) but not in an ependymal cell (a red arrow) after doxycycline administration (l,m). Scale bar: 25 μm in (b).