Figure 1 | Scientific Reports

Figure 1

From: Heterogeneous Porphyromonas gingivalis LPS modulates immuno-inflammatory response, antioxidant defense and cytoskeletal dynamics in human gingival fibroblasts

Figure 1

(A) Representative 2-DE gel proteomic map of the cellular component of HGFs in response to heterogenous P. gingivalis LPS for 24 h. Cellular proteins were seperated via 2-DE, using 24 cm pH 3-10NL IPG strips and homogeneous 12% SDS-PAGE gels. The map was analysed using Image Master 2D platinum software (version.7.0) (Amersham Biosciences). The molecular weight (MW) scale was constructed from protein standards (Bio-Rad Rainbow markers) run alongside the focused strip and the pI scale was constructed from the dimensions of the nonlinear pH gradient strips. The map shows the locations of all differntially expressed proteins that were significantly altered by P. gingivalis LPS treatment. Labeled protein spots with arrows were equivalent to the gene names specified. Each spot name relates to the data shown in Table S1. (B) Significantly up and/or down-regulated proteins in HGFs cellular proteome in response to both P. gingivalis LPS1690 and LPS1435/1449. The density of the protein spots in LPS-treated gels was normalized to that of the corresponding control group. Proteins that upregulated (≥2 folds) (B1) and downregulated (≤0.5 folds) (B2) in P. gingivalis LPS-treated groups as compared to the control are shown. Note that proteins labeled with lower case letters (a–c) represent isoforms from the same protein listed in Table S1. (C) Significantly upregulated proteins in P. gingivalis LPS1690 treated cellular proteome. The density of the protein spots in the P. gingivalis LPS-treated gels was normalized to the density of the corresponding control group. (D) Significantly up- or down-regulated proteins in P. gingivalis LPS1435/1449 treated cellular proteome. Significantly upregulated proteins (≥2 folds) (D1) and downregulated proteins (≤0.5 folds) (D2) in P. gingivalis LPS1435/1449 treated groups are shown. The density of the spots in the LPS treated gels was normalized to that of the corresponding control group. Annexin A2 (ANXA2), which was uniquely expressed in P. gingivalis LPS1435/1449 treated cells, is shown as two isoforms (D3).

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