Figure 4 | Scientific Reports

Figure 4

From: Heterogeneous Porphyromonas gingivalis LPS modulates immuno-inflammatory response, antioxidant defense and cytoskeletal dynamics in human gingival fibroblasts

Figure 4

The gene expression of CDH11, PPIA, CTSB and CST3 proteins in P. gingivalis LPS- and E. coli LPS-stimulated HGFs analysed by Western blot.

Confluent cells were treated with P. gingivalis LPS and E. coli LPS (1 μg/mL) for the indicated time points. 40 μg of homogenized, cellular extracts, were subjected to SDS-PAGE and probed with polyclonal antibodies against CDH11 (1:250), PPIA (1:1000), CTSB (1:1000) and CST3 (1:1000). Blots were reprobed with α-Tubulin to confirm the equal loading in individual samples. (A)The fold increase values of proteins as compared with α-Tubulin are shown in the graphs (arbitrary units over control after normalization to the total protein). (B) One representative blot is shown from three independent experiments with similar results. *Significant difference with a P-value <0.05 as compared with the controls without LPS treatment.

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