Figure 3: Macrophages are the major cell-type-responsive to IL-33 in spleen and liver granuloma of S. japonicum infected mice.

(a) Increased expression of ST2L on splenocytes and liver MNCs from S. japonicum-infected mice. Splenocytes and liver MNCs were isolated from the infected mice at week 0, 4 and 7 post-infection and detected ST2L by flow cytometry. High levels of ST2L were expressed on cells of mice infected with S. japonicum. The percentages of positive cells are indicated at the upper left of each chart. (b) ST2L+ cells could be expressed on the surface of different cell populations, including, F4/80+ macrophages, CD3+ T cells, CD19+B cells and CD19-CD3-NTNB cells. High levels of ST2L were expressed on the surface of macrophages. The flow cytometry data are representative of two independent experiments; *p < 0.05 and **p < 0.01, by the Student t test.