Figure 5
Isothermal titration calorimetry (ITC) thermograms recorded for injection of 10 mM CaCl2 into alginate, CS, alginate/CS mixed solutions and the corresponding binding isotherms according to molar ratio.
CaCl2 was injected into 2.52 mM alginate solution (Ca/alginate) (a), into 0.99 mM CS solution (Ca/CS) (b), into the solution containing 2.52 mM alginate and 0.25 mM CS (mass ratio of alginate and CS 4:1) (Ca/alginate-CS 4:1) (c) and the solution containing 2.52 mM alginate and 0.66 mM CS (mass ratio of alginate and CS 3:2) (Ca/alginate-CS 3:2) (d). Data showed that Ca2+ also could bind to CS chains, except for alginate chains.
