Figure 8
Higher metastatic potential of tumor cells cultured in ALG-CS on Day 7 compared with in ALG.
(a,b) Relative gene expression of MMPs in SAS and LM3 cells cultured in both beads as detected by quantitative real-time PCR. (c) Secreted MMP2 and MMP9 evaluated by zymography. (d,e) The cell number invaded through Matrigel-coated filter membrane was statistically analyzed. Adhesion cells cultured in T-flasks served as the controls (2D). Data are represented as the means ± SD of three independent experiments. *P < 0.05 and **P < 0.01.
