Figure 2: The CD82 scaffold regulates PKCα association with the membrane.
From: Tetraspanin CD82 Regulates the Spatiotemporal Dynamics of PKCα in Acute Myeloid Leukemia
(A–D) Flow cytometry analysis indicates the percentage of GFP-PKCα expression in transiently transfected cells. (E–H) Epifluorescence imaging of transfected cells showing GFP-PKCα localization +/− PMA. (I–L) PKCα trajectories from 600 frames of analyses are displayed. (M) Cumulative distribution plot of PKCα track length (n ≥ 31227 tracks from n ≥ 19 cells of each kind; the Kolmogorov-Smirnov test was used to compare cumulative distributions). (N) Average GFP-PKCα track length per cell and (O) per experiment (error bars indicate SD; n ≥ 19 cells, n = 3 experiments; post-hoc unpaired t-test). (R) Average track length per cell was quantified in control and CD82KD cells (error bars indicate SD; n = 22 cells).
