Figure 4: PKCα clustering at the membrane is controlled by the CD82 scaffold.
From: Tetraspanin CD82 Regulates the Spatiotemporal Dynamics of PKCα in Acute Myeloid Leukemia
Control, CD82OE and Palm-CD82OE KG1a cells were treated with DMSO, or PMA for 5 mins or 1 hr and imaged for PKCα (abcam, Y124; Invitrogen, rabbit-647) using dSTORM. (A) The DBSCAN algorithm was used to examine cluster organization within a subregion of the cells. Clustered localizations are indicated by color, whereas gray localizations did not meet the clustering parameters (ε = 50 nm, n = 30 localizations). The DBSCAN algorithm was used to determine the (B) number of PKCα clusters, (C) PKCα equivalent cluster diameter, and (D) PKCα molecular density (n ≥ 4 cells of each condition, error bars indicate SD, post-hoc unpaired t-test). CD82KD cells were transfected with GFP-PKCα and imaged using dSTORM. (E) PKCα clustering was quantified using the DBSCAN clustering algorithm in cells treated with DMSO or PMA (ε = 50 nm, n = 10 localizations). (F) The number of clusters (n = 7 cells, error bars indicate SD) and (G) the cluster diameters were quantified (n ≥ 561 clusters, error bars indicate SEM, unpaired t-test).
