Figure 5: CD82 modulates ERK1/2 activity downstream of PKCα stimulation.
From: Tetraspanin CD82 Regulates the Spatiotemporal Dynamics of PKCα in Acute Myeloid Leukemia
(A) Control, CD82OE and Palm-CD82OE cells were treated with DMSO or PMA for 1 hr and analyzed by Western blot analysis for total (D13E1) and phospho-p38 (Thr180/Tyr182). Representative Western blot showing control and (B) CD82OE cells or (C) Palm-CD82OE cells treated with PMA for 0, 5, 15, or 60 mins and analyzed for total ERK1/2 (137F5) expression. Representative Western blot depicting (D) control and CD82OE or (E) Palm-CD82OE cells treated with PMA for 0, 5, 15, or 60 mins and analyzed for phospho-ERK1/2 (Thr202/Thr204) expression. (F) Graphical depiction of phospho-ERK expression over time quantified by Western blot analysis. (n ≥ 4 experiments, error bars depict SEM; post-hoc unpaired t-test).
