Figure 6: CD82 regulates AML colony formation in a PKCα-dependent manner.
From: Tetraspanin CD82 Regulates the Spatiotemporal Dynamics of PKCα in Acute Myeloid Leukemia
(A,B) Control, CD82OE and Palm-CD82OE cells grown in clonogenic assays in the presence of PMA alone (10 ng/ml), or PMA + FR180204 (100 μM), or equal volumes of DMSO and assessed after 14 days by microscopy for the number of leukemia colony-forming units per 96 mm2 (n ≥ 4 experiments, error bars indicate SD). (C) Proposed model whereby the scaffolding function of CD82 regulates the membrane clustering and stabilization of PKCα, which controls ERK1/2 signaling and AML colony forming potential.
