Figure 4: Extended-depth SHG-2PEF imaging of a fibrotic kidney histological section (see also Supplementary movie).
From: Efficient second-harmonic imaging of collagen in histological slides using Bessel beam excitation
(A) 15 μm thick histological section of fibrotic mouse kidney was imaged with Bessel excitation (800 nm, 23 μm × 0.2 μm) and XY mosaicking. (A) Stitched 2D Bessel image of the entire histological section. SHG signal is shown in blue and endogenous 2PEF is shown in red. Imaged area: 5 × 5.5 μm2. Lateral pixel size 0.5 × 0.5 μm2. Pixel dwell time, 30 μs. Power after objective, 45 mW. Signal level up to 250 photons/pixel. Scale bar, 1 mm. (B) Zoomed-in area showing the local SHG and 2PEF contrasts. Scale bar, 50 μm. (C) Integrated radial profiles of the SHG signal (red) and of the square root of the SHG signal (black).
