Figure 5: 2PEF and SHG signal level for point-like and axially extended objects as a function of Bessel beam extension ρ. | Scientific Reports

Figure 5: 2PEF and SHG signal level for point-like and axially extended objects as a function of Bessel beam extension ρ.

From: Efficient second-harmonic imaging of collagen in histological slides using Bessel beam excitation

Figure 5

(A) Excitation PSF2 (square intensity distribution) estimated from camera-based measurements. Bessel beams parameters: 1100 nm, NAB = 1.05, 7 μm × 0.3 μm and 14 μm × 0.3 μm extensions. (B) Images of point-like (150 nm KTP crystal and 1 μm fluorescent polystyrene beads) and axially extended objects (Starch granule and 5% bodipy solution) recorded with the two beams. Pixel dwell times: bead, 20 μs; KTP, 10 μs; bodipy, 5 μs; starch, 50 μs. (C) Ratio between the signals obtained with the two beams for 2PEF and SHG, and for each type of sample.

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