Figure 3

Pseudomonas spp. used in the experiments.

(a), phylogenetic relationships between the genotypes used in the in vitro experiments and other well known biocontrol genotypes based on maximum likelihood tree estimated on a 940 kb fragment of the universal houskeeping gene gyrB, using the RAxML algorithm (with GTR-GAMMA substitution). Scale bar represents nucleotide substitutions per site. Blue strains are known biocontrol agents, red strains were previously isolated from wheat root systems and have been implicated in take-all control, grey strains are outgroups³⁸. (b), Take-all pathogen inhibition and bacterial antagonistic ability of the strains. Take-all pathogen inhibition is the area of fungal mycelial growth inhibited by each Pseudomonas genotype in Petri dish assays (1 = no fungal growth and 0 = complete fungal growth). Bacterial antagonism is the log ratio of strain productivity (OD ₆₀₀) on spent liquid medium cultured by its own genotype versus its mean productivity on spent medium cultured by all other genotypes. Data show means ± s.e.m. n = 3.