Figure 1 | Scientific Reports

Figure 1

From: Deregulation of the OsmiR160 Target Gene OsARF18 Causes Growth and Developmental Defects with an Alteration of Auxin Signaling in Rice

Figure 1

Expression analysis of OsMIR160a, OsMIR160b and OsmiR160 potential target OsARFs.

(a,b) Quantitative real time RT-PCR (qRT-PCR) results showing expression of OsMIR160a (a) and OsMIR160b (b). (c–f) qRT-PCR) results showing expression of OsmiR160 potential target genes: OsARF8 (c), OsARF10 (d), OsARF18 (e) and OsARF22 (f). S: seedling (7-day old), L: leaf, ST: stem, YI: young inflorescence and MI: mature inflorescence. Gene expression levels in other organs were normalized based on expression observed in seedling. Stars indicate significant difference (P < 0.01). (g) An unrooted phylogenetic tree constructed by the Maximum-Likelihood method showing miR160 target ARFs in Arabidopsis and rice. (h) The 5′ RACE result showing that OsARF18 is a target of OsmiR160. The arrow points to the OsmiR160-directed cleavage site at the OsARF18 transcript.

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