Figure 2 | Scientific Reports

Figure 2

From: Deregulation of the OsmiR160 Target Gene OsARF18 Causes Growth and Developmental Defects with an Alteration of Auxin Signaling in Rice

Figure 2

Generation of transgenic rice plants expressing an OsmiR160-resistant version of OsARF18 (mOsARF18).

(a) Domain structure of the OsARF18 protein. The B3 DNA binding domain (B3) and the C-terminal dimerization domain (CTD) are indicated in black and gray, respectively. The OsmiR160 complementary (or target) sequence in the OsARF18 mRNA and the corresponding region of amino acid sequence (GIQGAR) are shown. The silent mutations were created in mOsARF18 by introducing silent mutations (indicated by*). (b) qRT-PCR results showing that expression levels of un-cleaved OsARF18 were significantly (*P < 0.01) increased in leaf, stem and inflorescence of three examined independent mOsARF18 transgenic lines.

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