Figure 2: 3D architectures within a chloroplast in a live WT P. patens protonemal cell.
From: Live-cell visualization of excitation energy dynamics in chloroplast thylakoid structures
(a) 3D image of WT P. patens chloroplasts observed using SCLIM. The 3D image was reconstructed using 52 optical sections (x, y) sequentially acquired at 0.05 μm intervals (z = 2.6 μm). (b) Optical sections of a granum (shown in the square in (a)) from top to bottom. Number indicates slice numbers. Arrowheads indicate apparent stroma lamellae structures extending from grana margins at different positions. (c) Side views of the same granum structure (square in (a)) from different angles of the vertical cross section (A–C). Arrowheads labeled S1–S4 indicate the same structures as those in (b). The length indicates the scale of each unit. Scale bars are 1 μm (b,c).
