Figure 6: The expression of chemokines and the activation of NF-κB. | Scientific Reports

Figure 6: The expression of chemokines and the activation of NF-κB.

From: Tumourigenicity and Immunogenicity of Induced Neural Stem Cell Grafts Versus Induced Pluripotent Stem Cell Grafts in Syngeneic Mouse Brain

Figure 6

(a) The expression of chemokine-related genes, including Ccl5/7/11 and Cxcl1/2/5/12/16, was analyzed after signal values normalization (n = 3/group; ((a) P < 0.05 versus NSCs or iNSCs; (b) P < 0.05 versus iNSCs). (b,c) CCL5 and CXCL12 levels in the culture supernatants of these stem cells were detected by ELISA (n = 6/group; (a) P < 0.05 versus NSC, iNSC or MSC; (b) P < 0.05 versus NSC or MSC). (d,e) Ccl5 and Cxcl12 gene expression in brain tissues after stem cell transplantation was analyzed by qRT-PCR (n = 3/group; ((a) P < 0.05 versus ESC (G2), iPSC (G2), ESC (G1), iPSC (G1), ESC (NT), iPSC (NT), NSC, iNSC, MSC or Control; (b) P < 0.05 versus ESC (G1), iPSC (G1), ESC (NT), iPSC (NT), NSC, iNSC, MSC or Control; (c) P < 0.05 versus ESC (NT), iPSC (NT), NSC, iNSC, MSC or Control). (f) Representative immunoblots depicted the levels of CCL5, CXCL12, p65, p65 phosphorylated at Ser276 and Ser536 in the brain. (g-m) Histograms showed that the levels of CCL5 (g), CXCL12 (h), p65 (i), p65 phosphorylated at Ser276 (j) and Ser536 (k) in the brain, serum CCL5 (l) and CXCL12 (m) (n = 3/group (WB), n = 6/group (ELISA); ((a) P < 0.05 versus ESC (G2), iPSC (G2), ESC (G1), iPSC (G1), ESC (NT), iPSC (NT), NSC, iNSC, MSC or Control; (b) P < 0.05 versus ESC (G1), iPSC (G1), ESC (NT), iPSC (NT), NSC, iNSC, MSC or Control; (c) P < 0.05 versus ESC (NT), iPSC (NT), NSC, iNSC, MSC or Control).

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