Figure 1
The diurnal variation in the morphological and dynamic behavior of the microglial processes.
(a) The diurnal morphological variation in the intracellularly labeled cortical microglia. The Iba1-stained microglia (Iba1), intracellularly labeled microglia (lucifer) and their merged CLMS images. Scale bar: 10 μm. (b,c) The mean total length of the processes (b) and branch points (c) of the intracellularly labeled microglia at ZT2 and ZT14. The data are presented as the mean ± S.E.M. (N = 3 mice, n = 12–13 microglia each). A two-tailed unpaired t-test; ***p = 0.0005 (b), ***p = 0.0005 (c,d) The mean ratio of the total microglial process length of Iba1-immunostaind microglia to the intracellularly labeled microglia. The data are presented as the mean ± S.E.M. (N = 3 mice, n = 5 microglia each). A two-tailed unpaired t-test; p = 0.0325.
