Figure 4: Multiple alignment of protein sequence and phylogeny of the four cloned FIP-Lrh cDNA with reported FIPs. | Scientific Reports

Figure 4: Multiple alignment of protein sequence and phylogeny of the four cloned FIP-Lrh cDNA with reported FIPs.

From: Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis

Figure 4

The alignment is performed with the Clustal Omega (1.2.1) (EMBL-EBI) using the published FIP sequences of FIP-gja (G. japonicum) (GenBank: AAX98241.1), FIP-gsi (Ganoderma sinense), FIP-glu (G. lucidum) (UniProtKB/Swiss-Prot: P14945.2), FIP-gap (G. applanatum, GenBank: AEP68179.1), FIP-fve (Flammulina velutipes, GenBank: ADB24832.1), FIP-tvc (Trametes versicolor) and FIP-vvo (Volvariella volvacea) and gaps are introduced for optimal alignment and maximum similarity between all compared sequences. The identical amino acids among all the aligned sequences are indicated as ‘*’ whereas “:” conserved substitutions and empty space represents a non-conserved substitution. and the identical amino acids with FIP-gsi are shown in a gray background. The key residues in FIP-fve (W24, T28, D34, T90, I91 and W111) and the corresponding residues in FIP-Lrh (W25, D35, I92 and W112) present in the carbohydrate binding module (CBM) are shown as a solid line (). A Neighbour-joining phylogram (with real Branch length, distance corrected) was generated using the Omega Clustal program with FIPs from the representatives of genera Lignosus, Ganoderma, Flammulina, Volvariella and Trametes.

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