Figure 1 | Scientific Reports

Figure 1

From: Detection of influenza A virus from live-bird market poultry swab samples in China by a pan-IAV, one-step reverse-transcription FRET-PCR

Figure 1

Alignment of the primers and probes of the pan-IAV PCR with the IAV M gene sequences.

The sequences of the forward/reverse primers and the fluorescein/LCRed 640 probes are shown at the top of the boxes. The forward primer and two probes were used as the demonstrated sequences without gaps while the reverse primer was used as an antisense oligonucleotide. The 6-FAM label was attached directly to the 3-terminal nucleotide of the fluorescein probe and the LCRed 640 fluorescein label was added via a linker to the 5′-end of the LCRed 640 probe. Dots indicate nucleotides identical to the primers and probes. The designed oligonucleotides show minimal mismatches with influenza A virus (0 mismatch with 52 IAV subtypes, 1 mismatch with 13 subtypes, 2 mismatches with 2 subtypes and 4 mismatches with 1 subtypes).

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