Figure 1 | Scientific Reports

Figure 1

From: Impact of structural polymorphism for the Helicobacter pylori CagA oncoprotein on binding to polarity-regulating kinase PAR1b

Figure 1

In vitro reconstitution of CagA-PAR1b interaction.

(a) Schematic diagram of GST-PAR1b (39–364), which contains the catalytic and the UBA domains and CagA-2CMW (H. pylori 26695 strain) which contains 2 CM motifs: one in the EPIYA-C segment and the other immediately downstream of the segment (top). Results for the GST pull-down between GST-PAR1b (39–364) and CagA-2CMW (bottom). (b) Schematic diagram of PAR1b (39–364) and GST-CagA-2CMW (top) and the results of the GST pull-down using these constructs (bottom). (c) Schematic diagram of GST-PAR1b (39–364) and GST-CagA-2CMW constructs with CM deletions (left) and the results of the GST pull-down using these constructs (right). All pull-downs were performed in HBS-P buffer. Samples were resolved on SDS-PAGE gels and visualized by CBB.

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